Hanging Drop Tissue Culture
نویسنده
چکیده
The hanging drop tissue culture is a technique utilized in embryology [4] and other fields to allow growth that would otherwise be restricted by the flat plane of culture dishes and also to minimize the surface area to volume ratio, slowing evaporation. The classic hanging drop culture is a small drop of liquid, such as plasma or some other media allowing tissue growth, suspended from an inverted watch glass. The hanging drop is then suspended by gravity and surface tension, rather than spreading across a plate. This allows tissues or other cell types to be examined without being squashed against a dish.
منابع مشابه
Media additives to promote spheroid circularity and compactness in hanging drop platform.
Three-dimensional spheroid cultures have become increasingly popular as drug screening platforms, especially with the advent of different high throughput spheroid forming technologies. However, comparing drug efficacy across different cell types in spheroid culture can be difficult due to variations in spheroid morphologies and transport characteristics. Improving the reproducibility of compact...
متن کامل384 hanging drop arrays give excellent Z-factors and allow versatile formation of co-culture spheroids.
We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture and the importance of utilizing 3D cellular models in anti-cancer drug sensitivity testing. The 384 hanging drop array plate allows for high-throughput capabilities and offers significant improvements over existing 3D spheroid culture methods. To allow for practi...
متن کاملDifferentiation Patterns of Immortalized Human Meibomian Gland Epithelial Cells in Three-Dimensional Culture.
Purpose To establish a simplified three-dimensional (3D) meibomian gland culture model using a meibomian gland epithelial cell (HMGEC) line that might be a useful tool to gain deeper insights into meibomian gland dysfunction. For this purpose, 3D differentiation patterns and growth characteristics of HMGECs were studied on various membranes/scaffolds as well as in hanging drops. Methods Sever...
متن کاملColony, hanging drop, and methylcellulose three dimensional hypoxic growth optimization of renal cell carcinoma cell lines
Renal cell carcinoma (RCC) is the most lethal of the common urologic malignancies, comprising 3% of all human neoplasms, and the incidence of kidney cancer is rising annually. We need new approaches to target tumor cells that are resistant to current therapies and that give rise to recurrence and treatment failure. In this study, we focused on low oxygen tension and three-dimensional (3D) cell ...
متن کاملThe body-on-a-chip concept: possibilities and limitations
Recently, Frey et al. (2014) have established a reconfigurable microfluidic platform to study multi-tissue interactions. This platform contains multiple spheroids of different cell types in hanging drops. The hanging drops are connected by microfluidic networks. The path of the liquid flow through the hanging drops is precisely controlled and offers the possibility to perfuse them either sequen...
متن کامل